• Volume 40,Issue 8,2016 Table of Contents
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    • Research on the subcellular fate and transformation of cadmium in different tissues of the scallop Chlamys farreri

      2016, 40(8):1203-1210. DOI: 10.11964/jfc.20150609905

      Abstract (2020) HTML (581) PDF 1.38 M (1704) Comment (0) Favorites

      Abstract:In order to explain the high capability of the scallop Chlamys farreri for cadmium accumulation, the subcellular distribution of Cd in the different tissues of the scallop C. farreri and its relationship between subcellular distribution and metal biokinetics was investigated. The results showed that for the control group, the metallothionein-like protein (MTLP) and the cell organelles were the main detoxification pools for the digestive gland, gills and gonad, and MTLP was the main pool for the adductor muscle. During the accumulation experiment, for the digestive glands and gills, the content and the percentage of Cd in the MTLP and the metal-rich granule(MRG) were all significantly increased, but the percentage of Cd in the cell organelles decreased. The Cd content in the MTLP, cell organelles and MRG in the gonad were all significantly increased, but the percentage of Cd in the MTLP and cell organelles kept decreasing with the percentage of Cd in the MRG increased. For the adductor muscle, when exposed to Cd, the Cd content in the MTLP and MRG significantly increased, and the percentage of Cd in the MRG was also significantly increased. During the elimination experiment, for the digestive glands and gills, the content of Cd in the MTLP was significantly decreased, at the same time, the content of Cd and the percentage of Cd in the cell organelles, heat-sensitive protein (HSP) and MRG kept increasing; for the gonad, the Cd content in every subcellular fractions decreased obviously, but the percentage of Cd in different subcellular fractions had no obvious change. For the adductor muscle, Cd content in the MTLP and MRG significantly decreased, and the percentage of Cd in MRG also kept decreasing. Present results first disclosed that the subcellular distribution of Cd was different for different tissues in the scallop C. farreri, and the redistribution of Cd in different subcellular fractions in the digestive gland and gills occurred during Cd accumulation and elimination.

    • Statolith microstructure analysis of the Humboldt squid (Dosidicus gigas) in the eastern Pacific Ocean

      2016, 40(8):1211-1217. DOI: 10.11964/jfc.20150109673

      Abstract (1976) HTML (601) PDF 20.18 M (1404) Comment (0) Favorites

      Abstract:This study analyzed the growth increments, checks and aberrant structures of statolith microstructure for Dosidicus gigas in Costa Rican, Peruvian and Chilean waters in the eastern Pacific Ocean. The growth increments of the statolith are composed of one light and one dark circle line. Three different growth zones were defined bases on the width of growth increment that is postnucelar, dark and peripheral zone. The average age of paralarvae is about one month with 26 days in Costa Rica, 32 days in Peru and 33 days in Chile. Average age of juvenile is about 3 months with 86 days in Costa Rica, 84 days in Peru and 88 days in Chile. There were seven kinds of checks within the statolith, the formation of which might result from endogenous rhythm (such as hatching, mating and spawning) or external stress (such as thermal shock and attacking from predator). In addition, we also found several abnormal structures (such as additional focus, increment and center) and aberrant increment growth pattern in a few statoliths, which was believed to be the result of the damage of statolith caused by external stress.

    • Distribution pattern of fish community in Northern Jiangsu shoal in relation to bathymetry and tidal creek of sand ridge

      2016, 40(8):1218-1228. DOI: 10.11964/jfc.20150609914

      Abstract (1979) HTML (559) PDF 2.14 M (1348) Comment (0) Favorites

      Abstract:Based on the data from scientific fishery investigation in the waters of middle Northern Jiangsu shoal in May 2011 and November 2010, respectively, using the methods of variance and regression, the effects of bathymetry of sand ridge and tidal channel on the distribution pattern of the fishes were studied, through the analysis of the fish shape features, fish density and the index of relative importance of dominant species and the contribution of dominant species to the total population. Spatially, the distribution patterns of fish were associated with the bathymetry of the tidal channel system. The outer water of the tidal channel in spring was dominated by marine species, including Larimichthys polyactis and Michthys miiuy which migrated to the waters for spawning. Inside the tidal channel, Pholis fangi was predominant migratory fish, M. miiuy was also found in the area. In the sand ridge, it was mostly composed of adult of Thryssa kammalensis and no migratory fishes are found. In autumn, the distribution of fish species is only associated with the terrain of the bathymetry of the tidal channel system, in which it is almost composed of Protosalanx chinensis for shield in autumn. The result also shows that seasonal variation in densities of fish was closely associated with the spawning and nursery of fish community. The density of fish in terms of weight in spring (16.08×103 ind/km2) was higher than that in autumn (10.74 kg/km2). However, the density in terms of individual in spring (5.36×103 ind/km2) was lower than that in autumn (16.3×103 ind/km2). Both average weight and body length of fish in spring were greater than those in autumn, which was probably associated with immigration of the adult of fish to the studied area to spawn during spring (e.g., spawning community of L. polyactisr and Eupleurogrammus muticus). In autumn, it was mainly composed of foraging juveniles (e.g., M. miiuy and P. fangi), due to partial fishes' finding shelter and feed here. In general, because of the special terrain of North Jiangsu shoal, the sea area becomes the site of spawning in spring while the nursery field in summer and autumn, which is of great significance in fisheries.

    • Effects of culture media on the biofilm formation and subsequent settlement of Mytilus coruscus

      2016, 40(8):1229-1238. DOI: 10.11964/jfc.20160310318

      Abstract (2460) HTML (497) PDF 5.25 M (1538) Comment (0) Favorites

      Abstract:In order to determine the interactions among the biofilm characteristics, culture media and settlement of marine invertebrate, the present study investigated the effects of the biofilm formation in two types of culture media, and the subsequent effects of bacterial biofilms on the settlement and metamorphosis of Mytilus coruscus larvae. The biofilm inducing activities on larval settlement and metamorphosis were correlated to the bacterial density with exception of one and four species in culture media Zobell 2216E and Seawater Luria-bertani (SWLB), respectively. The inducing activity of the bacterial biofilms was correlated to the culture medium type and initial cell density of bacteria. Scanning electron microscope showed that a thick Staphylococcus sp. 3 biofilm comprising of a stack of bacterial cells under the condition of 2216E compared to SWLB. In contrast, patchy monolayer was found under 2216E in Pseudoalteromonas sp. 8 biofilm, and short rod was observed under SWLB. SDS-PAGE showed that protein profiles of both Staphylococcus sp. 3 and Pseudoalteromonas sp. 8 biofilms changed distinctly in two different culture media. In Staphylococcus sp. 3 biofilm, eight protein bands decreased significantly and two protein bands disappeared under SWLB compared to 2216E. In Pseudoalteromonas sp. 8 biofilms, five protein bands appeared under SWLB. Thus, the culture media impacted the development of bacterial biofilms such as distribution, morphology and phenotypes, and changes in biofilm characteristics may contribute to the variability in the inducing activity on larval settlement and metamorphosis.

    • Vertical distribution of the ions in overlying and interstitial waters and their diffusion fluxes in intensive hybrid snakehead aquaculture enclosures

      2016, 40(8):1239-1248. DOI: 10.11964/jfc.20140909461

      Abstract (2470) HTML (575) PDF 3.24 M (1366) Comment (0) Favorites

      Abstract:Intact overlying water and interstitial water in sediments were collected by Peeper devices from intensive hybrid snakehead (Channa maculata♀×C. argus) aquaculture enclosures in Nansha District, Guangzhou. The concentration of NH4+-N, NO3--N, NO2--N, PO43--P, SO42--S and Fe2+ were measured by miniaturised photometrical method using a microplate reader. All measured ions showed strong vertical distribution patterns, and the depth related samples were divided into three groups, overlying water group, surface layer sediment group (water-sediment interface group) and lower layer sediment group with distinct differences. Diffusion fluxes of the ions were calculated by the Fick's First Law. NH4+-N, NO2--N, PO43--P and Fe2+ were released from the sediment to overlying water, while NO3--N and SO42--S in the overlying water were absorbed into the sediment. Among them, NH4+-N and SO42--S have much higher exchange fluxes than the other ions relatively. And NH4+-N was the main endogenous pollution ions in the hybrid snakehead aquaculture enclosures with the diffusion fluxes of 76.432, 111.631 and 209.835 mg/(m2·d) respectively.

    • Identification, characterization and expression of NK-lysin in Megalobrama amblycephala

      2016, 40(8):1145-1155. DOI: 10.11964/jfc.20160110237

      Abstract (2087) HTML (530) PDF 14.27 M (1665) Comment (0) Favorites

      Abstract:NK-LYSINs are small cationic antimicrobial peptides produced by cytotoxic T lymphocytes and natural killer cells. Antimicrobial peptides, the important components of innate immune systems, have attracted scientists' attention because they can be served as novel antimicrobials. Megalobrama amblycephala is an important freshwater fish cultured in our country. A serious disease, bacterial septicemia caused by Aeromonas hydrophila is a big threat to M. amblycephala, which might lead to a high mortality. In order to understand the possible function of NK-lysin in the M. amblycephala immune system, we amplified and characterized the nkla and nklb genes in M. amblycephala, which encode 122 and 136 amino acids respectively. Sequences analysis showed that NK-lysin belong to the saposin-like protein family, with 6 highly conserved cysteines and a SapB domain. In different healthy fish tissues, the M. amblycephala nkla was highly expressed in the spleen, lowly in the muscle and blood, While nklb had the highest expression in the intestine and the relative lower level in the head kidney. However, after A. hydrophila infection, the M. amblycephala nkla and nklb shared the same expression trend. In the head kidney, the mRNA level decreased remarkablely at 4 h. In the liver, the expression increased to the peak significantly at 4 h and then fell down to the normal level. However, in the spleen and intestine, the expression fell to the bottom at 12 h and then reached the peak at 72 h. These results suggest that NK-lysin might play a vital role in the M. amblycephala defense against A. hydrophlia infection. What's more, we constructed prokaryotic expression system and obtained the recombinant proteins of M. amblycephala nkla and nklb, which may provide information for NK-lysin's further functional study.

    • The study on the proliferation of GCRVⅡ in different fish cell lines

      2016, 40(8):1249-1257. DOI: 10.11964/jfc.20160110256

      Abstract (2154) HTML (905) PDF 14.91 M (1563) Comment (0) Favorites

      Abstract:Currently, grass carp reovirus genotype Ⅱ(GCRVⅡ) are the leading cause of grass carp haemorrhage disease. To determine the optimum concentration of virus inoculation, various concentrations of HZ08 strain was inoculated with proboscis snout into fibers (PSF) cell of grass carp (PSF) and swim bladder cell of grass carp (GSB). Then we infected 10 kinds of fish cell lines with the optimum inoculation concentration, including liver cell of PSF, GSB, grass carp (L8824), ovary cell of grass carp (CO), etc. After inoculation, we observed the state of the cells with microscope daily, and real-time fluorescence quantitative PCR (qPCR) was used to analyze the proliferation of HZ08 in various cell lines. We also used indirect immunofluorescence to further qualitatively analyze the proliferation of HZ08 in various cell lines five days after infection. The results showed that the optimum inoculation concentration of HZ08 infected cells was 1.0×104 copies/μL. There was no significant cytopathic effect (CPE) observed in 10 kinds of fish cells inoculated with the HZ08 strain. qPCR analysis showed that HZ08 strain can proliferate in GSB, L8824, PSF, CF, CO, CIB, KS, 7 kinds of cells, which proliferated better in GSB, L8824 and PSF cells. The titers can reach 1.14×107, 5.90×106 and 6.30×104 copies/μL respectively. The result of immunofluorescence are coincided with the results of qPCR, there were strong fluorescence signals in GSB, L8824 and PSF cell lines and weak or even no fluorescence signals were observed in other cells. In conclusion, GSB, L8824 and PSF, these 3 kinds of cells are more sensitive cell lines for the proliferation of GCRVⅡ. In conclusion, this research is of great significance to the research of GCRVⅡ and the development of prevention and control product for GCRVⅡ.

    • Chromosome mapping using genomic DNA and repetitive DNA sequences as probes for somatic chromosome identification in Nibea albiflora

      2016, 40(8):1156-1162. DOI: 10.11964/jfc.20151110166

      Abstract (2199) HTML (535) PDF 13.19 M (1523) Comment (0) Favorites

      Abstract:Yellow croaker Nibea albiflora is a species of commercial important fish. However, chromosome identification is still difficult in yellow croaker for lack of cytogenetic markers. Hence, we analyzed the characteristics of chromosome in yellow croaker by using Giemsa staining, fluorescence staining, genomic in situ hybridization(GISH), and fluorescence in situ hybridization (FISH) with repetitive DNA sequences to assist chromosome identification. According to the distribution mode of fluorescent signal of self-GISH, 24 pairs of chromosomes could be distinguished and paired. FISH with 18S rDNA resulted in one pair of 18S rDNA signals distributing at the interstitial region of chromosome 1, which colocated with the secondary constriction after Giemesa staining, the negative band after DAPI staining, and the highlighted area after DPI staining. Whereas 5S rDNA FISH resulted in two pairs of signal with different intensity. The strong one was located on the centromere of chromosome 1, and the weak one was located on the distal position of chromosome 4. The signals of telomeric sequence were located on both termini of all chromosomes, although the signals of some chromosomes were weak. These results enriched the cytological genetic markers for chromosome identification in N. albiflora, and provided basic data for studying the chromosome evolution of Sciaenidae.

    • Preparation of the oral microencapsulated vaccine of Streptococcus agalaciate from tilapia and its immunological effect

      2016, 40(8):1258-1264. DOI: 10.11964/jfc.20160110264

      Abstract (2034) HTML (677) PDF 745.20 K (1788) Comment (0) Favorites

      Abstract:Desired for establishment of the prevention and control method of tilapia streptococcal disease, surface immunogenic protein (SIP) gene of Streptococcus agalaciate was prokaryotically expressed, and SIP fusion protein was used as core-material and natural polysaccharideⅡ as shell material. The oral polyacrylic resin microencapsulated vaccine of S. agalaciate was prepared. The average diameter of microencapsulated vaccine particles was 826.5 μm, the package rate was 72.02% and the amount of medicine loaded was 6.11%. Solubility of protein SIP oral microencapsulated vaccine in five pH conditions was pH 6.80>pH 7.20>pH 9.18>pH 4.68>pH 2.00. The in vitro experiment showed that oral microencapsulated vaccine releases protein SIP 5426.0 μg in simulated intestinal fluid, however, only 395.5 μg in simulated gastric fluid, and accumulative release rate was 90.51% in simulated intestinal fluid of 240 min. The vaccine could avoid destroying gastric acid and had good characteristics of intestines dissolution. Tilapias (Oreochromis niloticus) were immunized by oral microencapsulated vaccine. The antigens (SIP proteins) were respectively used at 5 μg and 10 μg antigens per gram of tilapia each time of immunization. After every immunization, a high level of IgM specific to SIP was detected with the antisera from the tilapia, the titer of 5 μg group was 100-1~400-1, and the titer of 10 μg group was 200-1~1600-1. The results indicated the fish had produced high titer antibody against S. agalaciate. After four times of immunization, tilapias were challenged by artificial infection with S. agalactiae and relative percent survivals (RPS) were calculated. The RPS of 5 μg group and 10 μg group were 44.45% and 66.67% respectively. The results showed that the oral microencapsulated vaccine was a convenient, safe and effective method for immune control of S. agalactiae in tilapia.

    • Principal component and path analysis of morphological traits of selective groups at different month ages of Sebastes schlegeli

      2016, 40(8):1163-1172. DOI: 10.11964/jfc.20150609924

      Abstract (2228) HTML (541) PDF 568.88 K (1683) Comment (0) Favorites

      Abstract:In order to research growth characteristics and the effect of morphological traits on body weight of selective groups of Sebastes schlegeli at different month ages, this thesis analyzed the data of body weight, full length, body length, head length, trunk length, body depth, caudal peduncle length and caudal peduncle depth at different month ages, by the methods of principal component, correlation analysis, path analysis and multiple regression analysis. The results showed that notable correlation was found among all the morphological traits of S. schlegeli at different ages, especially between full length and body length. The first principal component always reflects the length and body weight factor of S. schlegeli at different growth phases. However, the second principal component was different and reflected the trunk length factor at 15-month-old and the caudal peduncle factor at 18-month-old, but was not available at 12-month-old. The traits with strongest direct effect on body weight were body length and body depth (0.340, 0.314 and 0.347, 0.324) at 12-month-old and 15-month-old, meanwhile, the full body and body depth (0.498) at 18-month-old. The result of determinant coefficient analysis was consistent with that of path analysis. The growth rate of traits and effect on weight selected traits were not the same because of the different growth stages of S. schlegeli. We proposed that the body length and body depth were the target traits for selective breeding of S. schlegeli at 12-month-old and 15-month-old, We should pay attention to selecting full length at 18-month-age to improve the accuracy of breeding projects. The research provided theoretical evidence and technical parameters for selective breeding of S. schlegeli.

    • The mesh size selectivity of gillnets to Chelidonichthys spinosus in the East China Sea

      2016, 40(8):1265-1271. DOI: 10.11964/jfc.20150709969

      Abstract (2839) HTML (484) PDF 2.26 M (1659) Comment (0) Favorites

      Abstract:In order to find the selectivity of gillnets in the East China Sea, a production test using several gillnets of various mesh sizes (45,50 and 55 mm)and two gear heights (6 and 10 m)was carried out. Three uni-models (Normal, Lognormal and Gamma)were used to analyze the selectivity of the test nets. The parameters were estimated using maximum like lihood methods. Likelihood ratio Chi-square test and Akaike's information criterion (AIC) were used to compare the goodness of fit of the different models.Theresult showed that the fitting goodness of lognormal model is the best.The model is lower than, AIC is 147.78. The right tilt of the lognormal is obvious, the selectivity increased faster at the left side of the model peak, and gentle decline at the right side, which are well coincided with the original data.The 50% select body length of 50 mm mesh size is 123 mm, the select range is 123-209 mm. According to the population structure estimation which contacted test nets, the catch number of different body-length groups is normally distributed, the dominant range is 125-185 mm, this result is similar to previous studies. The select range of 50 mm mesh size covered the estimated dominant range, 50 mm mesh size is the most efficient for Chelidonichthys spinosus, but the influence of the mesh size on the fishery resources can not be evaluated due to the lack of knowledge about C. spinosus resources situation; the catches of the two heights test nets (6 and 10) are similar. The influence of panel heights to catches of C. spinosus is not obvious. These lesults will provide scientific guideline for coastal fishery management.

    • >ROUNDUPS
    • Studies of toxicity effects of heavy metals on early life stages of fish: a review

      2016, 40(8):1272-1288. DOI: 10.11964/jfc.20150709961

      Abstract (2158) HTML (680) PDF 39.10 M (2089) Comment (0) Favorites

      Abstract:The early life stages (ELSs) of aquatic organisms are particularly sensitive to both natural and anthropogenic stressors. ELS bioassays have widely been used as a cost-effective means to rapidly detect and monitor metal pollution in aquatic environments. Exposure to waterborne heavy metals during the ELSs of fish can result in inhibition of oogenesis and spermatogenesis processes, reduced fertilization success, chromosomal abnormalities, increased incidence of yolk membrane ruptures, reduced hatching success and survival, altered time to hatch, embryonic and larval teratogenicity, inhibited growth and abnormal larval behaviors. Previous studies have shown that heavy metals in dissolved and suspended particulate matters in the waters and sediments of fish spawning and nursery grounds in the offshore of China were overloaded in the past decades. Therefore, biological damage caused by heavy metals to their reproduction, development, survival, and growth have been considered potential risks for deterioration of the wild population. In this paper, studies of the toxicity effects on the ELS of fish were reviewed in order to help researchers better understand the progress in this research field.

    • >PAPERS
    • Path analysis for the correlations between morphological traits and body weight in five Macrobrachium nipponense populations

      2016, 40(8):1173-1185. DOI: 10.11964/jfc.20150709986

      Abstract (2595) HTML (532) PDF 858.93 K (1699) Comment (0) Favorites

      Abstract:In order to determine the influence of the main morphological traits on body weight of Macrobrachium nipponense, and to screen the optimal and measurable indices for artificial breeding of M. nipponense, the correlations between morphometric attributes and body weight of M. nipponense were explored using correlation analyses, path analyses and regression analyses. Ninety individuals from each of five populations (Tai Lake, Poyang Lake, Baiyang Lake, Weishan Lake and Dianshan Lake) were randomly sampled for the measurements of their body weight and 18 morphometric attributes, including the body length, overall length, rostrum length, second pereiopod length, diatance between eyes, carapace length, carapace width, carapace height, first abdominal segment height, first abdominal segment width, abdomen length, telson length, telson width, telson height, fantail length, fantail width, number of upper frontal eminencespin and number of lower frontal eminence spin. Correlation coefficients among all variables were calculated. Path coefficients were calculated by setting the body weight as a dependent variable and other attributes as independent variables via stepwise elimination. The path coefficients showed that: (1)five morphometric attributes of the Tai Lake population (body length, carapace width, second pereiopod length, carapace height, fantail length) significantly correlated to body weight. A multiple regression equation of the five attributes was established, where the regression intercept and regression coefficients were -9.878, 0.075, 0.314, 0.011, 0.320, 0.222, respectively. (2)seven morphometric attributes of the Poyang Lake population (carapace length, fantail length, telson width, carapace width, carapace height, telson height, body length) correlated significantly to body weight, where the regression intercept and regression coefficients were -1.618, 0.392, -0.280, 0.703, 0.524, -0.359, 0.688, -0.061, respectively. (3)four morphometric attributes of the Baiyang Lake population (body length, carapace width, second pereiopod length, fantail length) significantly correlated to body weight, where the regression intercept and coefficients were -4.796, 0.082, 0.222, 0.007,0.136, respectively. (4)four morphometric attributes of the Weishan Lake population (carapace length, first abdominal segment height, second pereiopod length, first abdominal segment width) significantly correlated to body weight, where the regression intercept and coefficients were -7.644, 0.248, 0.329, 0.025, 0.301. (5)five morphometric attributes of the Dianshan Lake population(body length, second pereiopod length, carapace width, telson width, frirst abdominal segment width) significantly correlated to body weight, where the regression intercept and coefficients were -6.257, 0.019, 0.018, 0.164, 0.264, 0.162, respectively. It is obviously that the body length and carapace traits in different populations have great effect on the body weight.High multiple-correlation coefficients of the reserved morphometric traits on the body weight in each population were greater than 0.85 (Tai Lake R2=0.965, Poyang Lake R2=0.904, Baiyang Lake R2=0.902, Weishan Lake R2=0.971, Dianshan Lake R2=0.955). The main attributes of each population effecting the body weight were confirmed. The clustering results showed that the closer the geographical location, the more similar the shape in terms of the morphological traits.

    • Effects of LHRH-A2 on induced spawning and responses of plasma biochemical indices of Coilia nasus

      2016, 40(8):1186-1194. DOI: 10.11964/jfc.20151110146

      Abstract (2264) HTML (536) PDF 14.12 M (1490) Comment (0) Favorites

      Abstract:Effects of LHRH-A2 on induced spawning and plasma biochemical indices responses of Coilia nasus were studied in order to accomplish the artificial breeding protocol of this species. LHRH-A2 was used in those farm-raised, two-year-old (over 2 winters) C. nasus to analyze the function of induced spawning. Plasma biochemical indices of female C. nasus were determined using radioimmunoassay and chemiluminescent. Our results indicated that the average mortality rate of C. nasus was 16.67% ± 3.34% while female fish were injected a dose of 30 μg LHRH-A2/kg body mass and male fish were injected with a dose of the half of the amount for female fish. The time of oxytocin induction in those survival fish ranged from 21 to 24 h, the average spawning rate was 83.33% ± 3.34% and the average fertilization rate was 75.06% ± 6.19%. The concentration of plasma 17β- estradiol (E2), prolactin thyroxine (T4), cortisol, total protein (TP) and lactic acid (lactate) increased initially and decreased thereafter while the contents of triiodothyronine (T3), triglyceride (TG) and chloride ion (Cl-) decreased constantly and the contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), osmotic pressure, potassium (K+) and sodium (Na+) increased continuously within 21 hours after the LHRH-A2 was used. The results also indicated that high cortisol, total protein, triglycerides, osmotic pressure, sodium ions, and potassium ions plasma environment, and a low 17β- estradiol, prolactin, lactic acid, thyroxine, triiodothyronine, chloride, alanine aminotransferase and aspartate aminotransferase plasma environment occurred in those spawned C. nasus. The opposite results occurred in those not spawned individuals. In summary, the normal functions of liver and kidney of C. nasus were influenced by the manipulation of inducing spawning, which resulted in the fact that the regulation of oxytocin LHRH-A2 to egg maturation was restricted. This preliminary study revealed the effect of LHRH-A2 on induced spawning and responses of plasma biochemical indices of C. nasus and provided the basis for the commercially artificial breeding of C. nasus.

    • The developmental morphology of ovoviviparous Sebastes schlegeli in vitro culture

      2016, 40(8):1195-1202. DOI: 10.11964/jfc.20151010108

      Abstract (1965) HTML (589) PDF 14.76 M (1479) Comment (0) Favorites

      Abstract:In order to explore the developmental embryo morphology in vitro culture and design the most optimal embryo medium for Sebastes schlegeli, physiological saline as the main formula set as control group (first group) was respectively supplemented with 1% serum of S. schlegeli (second group), 1% homogenate of ovarian tissue of S. schlegeli (third group), 1% fetal bovine serum (fourth group), 1% serum of S. schlegeli and 1% homogenate of ovarian tissue of S. schlegeli (fifth group), which formed into five different media. High blastocyst stage and crystal stage were respectively set as the original embryonic phase, and then were placed in the above five different media. According to the observation and records, present study found that when the original embryonic stage was high blastocyst stage, although embryo in every group can develop to 16-sarcomere stage, developmental speed and duration greatly varied. The fifth group displayed fastest developmental speed for 60 h, the fourth groups show the worst performance for 67 h and the speeds of the rest of the groups were between the fourth group and fifth group; when the initial stage was crystal phase, the fifth had the fastest speed for 57 h and the highest hatching rate at 80%. The second possessed identical speed, but had lowest hatching ability for 40%. Research showed that, under the in vitro culture condition, it needed about 200 h to make fertilized eggs develop to newly hatched larvae and it was the fifth embryonic medium that made the embryo have fastest developmental speed in S. schlegeli.

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