• Volume 31,Issue 1,2007 Table of Contents
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    • Isozyme polymorphism in Portunus trituberculatus from wild popolation

      2007, 31(1):1-6. DOI: 10.3724/SP.J.1231

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      Abstract:Portunus triuberbuculatus is an important economical species in China. In recent years, the wild stocks of P.triuberbuculatus along the coast of China have suffered fatal decrease in their resources due to the overfishing and cultivation. So it is very important to investigate the genetic diversity of P.triuberbuculatus. In October 2005, 48 individuals were collected from Zhoushan Gulf. Muscle was collected from each of the samples and stored at -70 ℃ for detection. Muscle was homogenized in mercaptoethanol buffer(0.05%) at a ratio of 1∶3 (g·mL-1) with ice-bath and centrifuged at 12 000 r·min-1 for 30 min , 4 ℃. The supernatant was used as the isozyme source and electrophoresis was carried out on vertical polyacrylamide gels in Tris-gly buffer system (TG, pH 8.3) at 280 V for approximately 6 h at 4 ℃. The isozymes were analyzed by specific isozyme staining methods. The genetic variation was analyzed in terms of their locus number, structure, alleles, etc. Results showed that there were 20 loci by detecting eleven isozymes in this study. Among the scored loci, 4 loci (Me-2,Sod-3,Cat-3,Ldh-2) were polymorphic and the mean proportion of polymorphic loci (P) was 20%. The mean effective number of alleles per locus (Ae) was 1.230, and the mean expected heterozygosity per locus (He) and the mean observed heterozygosity (Ho) were 0.094 and 0.175, respectively. In the mean time, the deviation indexes from Hardy-Weinberg equilibrium (d) of four polymorphic loci (Me-2,Sod-3,Cat-3,Ldh-2) were analyzed with 1.000, 1.000, 0.818 and 0.333, respectively. The genetic variability of Portunus trituberculatus was higher than those of Eriochier japonica, Eriochier sinensis. 22 Refs.In Chinese.

    • Effects of 4-nonylphenol on expression of estrogen receptor and level of 17β-eatradiol in vivo of female Carassius auratus

      2007, 31(1):7-14. DOI: 10.3724/SP.J.1231

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      Abstract:The estrogen-like effects of 4-nonylphenol(4-NP)in vivo of female Carassius auratus were investigated in this paper. We gave intraperitoneal injection in female Carassius auratus with the dose of 1 mg·kg-1, 50 mg·kg-1, 100 mg·kg-1 of 4-NP or 1 mg·kg-1 of 17β-estradiol (E2) respectively. The expression of α-type estrogen receptor (ER-α) and CYP1A in liver was evaluated by RT-PCR and the concentration of E2 in serum was measured by fluorescent immunoassay in 24 h and 48 h of injection. The results show that 1 mg·kg-1 of E2 increased expression of ER significantly both in 24 h and 48 h (P<0.01). In 4-NP treatment group, the concentration of E2 was decreased and the expression of ER was increased with the increase of 4-NP’s level. In 1 mg·kg-1 of 4NP treatment group, there is a significant increase of expression of ER at 24 h (P<0.01) and 48 h (P<0.05), the concentration of E2 was decreased at 24 h (P<0.05) yet returned slightly at 48 h, and CYP1A’s expression is up-regulated at 24 h (P<0.05) and 48 h (P<0.01). In 50 mg·kg-1 of 4-NP treatment group, there is a significant increase of expression of ER at 24 h (P<0.01) and 48 h (P<0.05), the concentration of E2 was decreased significantly both at 24 h and 48 h (P<0.01), and CYP1A’s expression is up-regulated at 24 h (P<0.05) and 48 h (P<0.01). In 100 mg·kg-1 of 4-NP treatment group, there is a significant increase of expression of ER both at 24 h and 48 h (P<0.01), the concentration of E2 was decreased significantly at 24 h (P<0.05) and 48 h (P<0.01), and CYP1A’s expression is up-regulated (P<0.01). Effect of increasing of ER’s expression is E2>100 mg·kg-1 4-NP >50 mg·kg-1 4-NP >1 mg·kg-14-NP; effect of decreasing of concentration of E2 is 100 mg·kg-1 4-NP >50 mg·kg-1 4-NP >1 mg·kg-14-NP. The effect of 100 mg·kg-1 of 4-NP on ER’s expression is close to that of 1 mg·kg-1of E2, this indicates that 4-NP has weak estrogen-like effects. And 4-NP can up-regulate the expression of CYP1A (dose dependent). That increase in ER’s expression and decrease in E2 in 4-NP treatment group provide support of 4-NP’s endocrine disruption via ER pathways in Carassius auratus. 30 Refs.In Chinese.

    • Characteristics of enzymes extracted from abalone (Haliotis discus hannai Ino) intestine and purification of the alginate lyase

      2007, 31(1):15-22. DOI: 10.3724/SP.J.00001

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      Abstract:Foundamental Courses of Aquatic Products Science. Characteristics of enzymes extracted from abalone (Haliotis discus hannai Ino) intestine and purification of the alginate lyase. The enzymes extracted from abalone (Haliotis discus hannai Ino) intestine were studied for finding the alginate and cellulose degradation enzymes. The enzymes were separated and partially purified from abalone intestine by using ammonium sulfate precipitations, and successive fractionation on DEAE-52, SephadexG-200 column chromatography. The results showed that their were four types of enzyme separated, which were alginate lyase Ⅰ, lyase Ⅱ, cellulase and agarase. The characteristics of alginate lyase Ⅰ,lyase Ⅱ and cellulase related with pH, temperature and ions were also determined and the results indicated that alginate lyase Ⅰ has the maximum activity at pH 8.6 and 35 ℃. Alginate lyase Ⅱ has maximum activity at pH 7.2 and 35 ℃. Cellulase has maximum activity at pH 5.0 and 40 ℃. The alginate lyaseⅠ and lyase Ⅱ were not stable at high temperature. The crude enzymes exstracted could be obviously activated by Mn2+, Al3+,Ca2+,Cu2+ and inhibited by Na+. SDS-PAGE was used for estimating the molecular weights of enzymes and the findings showed that the molecular weights of alginate lyase Ⅰ, Ⅱ were to be 35.2 ku, 67 ku. The findings indicated that there were two types of alginate enzyme could be obtained from abalone(Haliotis discus hannai Ino) intestine and the studies established the purification steps and theoretical base for further work, reconstruction, production and application of three enzymes. Further work should be carried out on the N end and sequences of the enzymes. 12 Refs.In Chinese. Key words: Haliotis discus hannai Ino; alginate lyase; cellulose; purification KANG Ping(Department of Food Engineering, Dalian Fisheries University, Dalian116023, China) , WANG Qiu-kuan, SONG Lin-lin, XU Ling//J Fish China,2007,31(1).15-22

    • Feeding ecology of Thunnus obesus in the western Atlantic Ocean

      2007, 31(1):23-30. DOI: 10.3724/SP.J.1231

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      Abstract:Aquatic Resource Science. Feeding ecology of Thunnus obesus in the western Atlantic Ocean. Observation on feeding ecology was conducted based on the data of Thunnus obesus captured by tuna longline fishery in the western Atlantic Ocean from August, 2004 to March, 2005. The food items in stomach content of Thunnus obesus consist of Sardina pilchardus, Loligo pealei, Penaeus japonicus, Brama brama, Alepisaurus ferox , etc. However, these have a significant monthly variation, Sardina pilchardus is the majority of prey (mean= 55.77%) in each month and followed by Loligo pealei. The significant monthly variation of mean stomach fullness and percentage of Thunnus obesus with empty stomach have been observed. In the group of their fork lengths (FLs) less than 100 cm, the percentage of empty stomach is decreasing with the FL increasing; when their FLs fall into 100-150 cm, the percentage of empty stomach is increasing sluggishly; and when their FLs greater than 150 cm, the percentage of empty stomach shows an insignificant variation, mean stomach fullness has a decreasing trend with the FL increasing. Mean stomach fullness has a decreasing trend basically with the increasing of FLs, and monthly mean stomach fullness varies unremarkably and mostly remain to 0.35-0.40. Percentage weight of Sardina pilchardus in major prey groups of each FL class of Thunnus obesus is kept on the level of 50% and more. Monthly Shannon-Weiner diversity index H′of Thunnus obesus has an insignificant variation and maintains on the index H′ level of 1.6. The index H' is quite stable at the FL in 90-160 cm, and decreasing if the FL is longer than 160 cm. 22 Refs.In Chinese. Key words:western Atlantic Ocean; Thunnus obesus; feeding ecology; fork length; Shannon-Weiner diversity index H′ ZHU Guo-ping(College of Marine Science and Technology, Shanghai Fisheries University, Shanghai 200090,China), ZHOU Ying-qi, XU Liu-xiong, JIANG Wen-xin//J Fish China,2007,31(1).23-30

    • Effect of water current and temperature on growth of juvenile Acipenser baeri

      2007, 31(1):31-37. DOI: 10.3724/SP.J.00001

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      Abstract:Foundamental Courses of Aquatic Products Science. Effect of water current and temperature on growth of juvenile Acipenser baeri. Effect of water current and temperature on growth of juvenile sturgeon Acipenser baeri in intensive indoor (semi-recirculating) tank culture was investigated in a 90-day rearing trial. Fish were reared in continuous water current with three levels of velocity of 0.12(V1), 0.16(V2) and 0.20 m·s-1 (V3) respectively, and isochronic water current (in half an hour with a velocity of 0.20 m·s-1, then in the next half an hour of intermission with a velocity of 0 m·s-1)(V4), under water temperature varying between 13.8 ℃ and 24.7 ℃. Within a range of water temperature of 16.7 - 22.5 ℃, growth efficiency (GE) and daily growth rate (DGR) increased with the increase of velocity of water current, and maximum DGR occurred in V3 group. There was significant positive linear relation between GE and V: GE=52.5 V + 69.567 (R2 = 0.997, P< 0.05), when the velocity of water current ranged from 0.12 m·s-1 to 0.20 m·s-1. In contrast, within a range of water temperature of 13.8 - 16.7 ℃ or 22.5 - 24.7 ℃, DGR of V3 group were lower than that of V1 and V2 groups. Fish in the group with intermission of non-water current obtained the highest GE while lowest FCR at a water temperature of 20.7 ℃. It suggested that water current and water temperature have reciprocal effect on the growth of juvenile sturgeon A. baeri. The fish has different optimal water temperatures at different velocity of water currents, and the optimum temperatures are between 20.2 ℃ and 20.7 ℃.This result suggests that group V4 is the best among these groups that can produce better economic benefits. 22 Refs.In Chinese. Key words:Acipenser baeri; growth; intensive indoor (semi-recirculating) tank culture; current; temperature HUANG Ning-yu(East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai200090, China), CHENG Qi-qun, GAO Lu-jiao, YAO Zong-li, XIA Lian-jun//J Fish China,2007,31(1).31-37

    • Effects of temperature and shell length on the excretion of nitrogen and phosphorus of Chlamys farreri

      2007, 31(1):38-44. DOI: 10.3724/SP.J.1231

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      Abstract:Foundamental Courses of Aquatic Products Science. Effects of temperature and shell length on the excretion of nitrogen and phosphorus of Chlamys farreri. The excretion features of farrer’scallop (Chlamy farreri) under simulated natural conditions were studied with the aim of learning the modality of nitrogen and phosphorus excretion, and their excretion rates. Moreover, the investigation of the relationship among water temperature, shell length and alga concentration was also carried out, and some mathematics models about several factors were made. The results showed the excretion rates of DTN and PTN were 14.94 μg·(h·ind) –1 to 248.28 μg·(h·ind) -1 and 1.47 μg·(h·ind) -1 to 84.08 μg·(h·ind) -1,and the excretion rates of DTP and PTP were 0.007 μg·(h·ind) -1 to 42.54 μg·(h·ind) -1 and 0.916 μg·(h·ind) -1 to 17.78 μg·(h·ind) -1. The content of DTN and PTN were 77.2% and 22.8%, there into, the content of ammonia was 50.8%, while the contents of DTP and PTP were 75.6% and 24.4%. The individual total nitrogen and phosphorus excretion rates of the scallop are positively correlated with the shell length and temperature in range of 9.2-27.3 ℃. After considering dissolution and particle excretion of nitrogen and phosphorus, the excretion rates of C.farreri ware related to temperature by quadratic equations, and to shell length by the allometric equations. The interaction of temperature and shell length with DTN,PTN,DTP and PTP could be described by the models VDTN = 131.13Lnt+217.95LnL-617.65, VPTN=67.41Lnt+54.07LnL-242.37, VDTP=8.67Lnt+65.82LnL-112.15 and VPTP=11.73Lnt+11.14LnL-43.74, furthermore, the results of analysis covariance of those models were P<0.01. 22 Refs.In Chinese. Key words:Chlamys farreri; nitrogen; phosphorus; excretion SONG Xian-li(Yellow Sea Fisheries Research Institute of Chinese Academy of Fishery Science, Qingdao266071, China), SUN Yao, WU Jin-xuan, YIN Hui, XU Lin-mei//J Fish China,2007,31(1).38-44

    • Molecular cloning of hepatic glucose-6-phosphatase catalytic subunit from Erythroculter ilishaeformis: response of its expression to refeeding and carbohydrate in diet

      2007, 31(1):45-53. DOI: 10.3724/SP.J.1231

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      Abstract:Foundamental Courses of Aquatic Products Science. Molecular cloning of hepatic glucose-6-phosphatase catalytic subunit from Erythroculter ilishaeformis: response of its expression to refeeding and carbohydrate in diet. Liver glucose-6-phosphatase(G6Pase, EC 3.1.3.9),one of the key enzymes in the glycolysis and glyconeogenesis,plays a key role in blood glucose homeostasis by catalyzing the dephosphorylation of glucose-6-phosphate(Glu-6P) to glucose. RT-PCR and RACE(rapid amplification cDNA ends ) was used for the isolation of the full length cDNA of G6Pase gene from liver of Erythroculter ilishaeformis. The cDNA was 1913 bp containing the 49 bp 5′-untranslated region, 778 bp 3′-untranslated region and 1068 bp open reading frame, which encoded 355 amino acid with a predicted molecular weight of 39.89 ku. We compared the E.ilishaeformis alignment of deduced amino acid sequences of G6Pase cDNA with Danio rerio, Mus musculu, Canis familiaris, Homo sapiens, Xenopus laevis, Sparus aurata and Tetraodon nigroviridis. The score was 95%, 63%, 63%, 63%, 63%, 69 %, 55% and 76% respectively. E.ilishaeformi G6Pase also contained three conserved domains. To examine the relationship between dietary carbohydrate and G6Pase gene expression, we compared the G6Pase mRNA levels in different dietary with or without carbohydrate. Two groups of Erythroculter ilishaeformis were pair-fed for 8 weeks either a high protein without carbohydrate(63.38% protein,0 carbohydrate)or a low protein with carbohydrate (40.53%protein, 23.98%carbohydrate) diet. Real time RT-PCR was used to examine the levels of G6Pase mRNA in fasted and 3, 6, 12, 24 h after feeding in contrast to the different dietary. There was some increase in the level of G6Pase mRNA in dietary with and without carbohydrates at 12 h after feeding, which showed that refeeding affected the gene expression of G6Pase. Levels of G6Pase mRNA were 2-4 times in dietary with carbohydrate than those without carbohydrate at fasted and 3, 6, 12 h after feeding. These results showed that dietary carbohydrate can enhance the liver G6Pase gene expression. 21 Refs.In Chinese. Key words:RACE; G6Pase; carbohydrate; Erythroculter ilishaeformis; real time PCR TANG Yong-kai(Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences,Wuxi 214081, China) , YU Ju-hua, LIU Bo, GE Xian-ping //J Fish China,2007,31(1).45-53

    • The influences of xylanase added in wheat basal diet on intestine chyme viscosity and the development of villi and microvilli of Tilapia nilotica

      2007, 31(1):54-61. DOI: 10.3724/SP.J.00001

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      Abstract:Aquatic Feed Science. The influences of xylanase added in wheat basal diet on intestine chyme viscosity and the development of villi and microvilli of Tilapia nilotica. Wheat is important cereal crop in our country. Recently, because of the rise of science and technology, the output of wheat have increased significantly and exceeded in phase and structure. Meanwhile, the flourishing developments of aquatic feed industries have been restricted seriously by the defect of raw material. Using wheat as raw material of feed, carrying out transformation and consumption by aquaculture, all of these now possess the resources feasibility. The use of wheat in aquatic feed industry as major energy feed has been restricted because of xylan. In the past few years, the application of feed xylanase with wheat basal diet has resolved the problems of the anti-nutrition of xylan successfully. Xylanase could improve the growth of aquatic animals fed with wheat basal diet. But there weren’t any researches about the mechanism of xylanase promoting aquatic animals’growth at home and abroad. The purpose of adding xylanase to wheat basal diet in this paper was to study the influences of xylanase on intestine chyme viscosity, on the developments of intestine villi and microvilli. In this study we also probed into the relationship between intestinal environment and the developments of villi and microvilli, displayed the mechanism of xylanase relieving the anti-nutrition of xylan and promoting Tilapia nilotica’s growth. Wheat basal diet was control. The tested diets were wheat basal diet added with different levels of xylanase (0.05%, 0.10%, and 0.15% respectively). Each treatment was devised with 5 repeats and each repeat had 40 male Tilapia nilotica. The fish in floating cages were fed to satiation for 75 days. The results indicated that adding xylanase to wheat basal diet could decrease intestine chyme viscosity, promote the developments of villi and microvilli of Tilapia nilotica. The intestine chyme viscosity of 0.05%,0.10%,0.15% xylanase groups [(13.75±0.45) cps, (12.23±0.16) cps and (11.92±0.22) cps respectively] was significantly lower than that of control [(15.21±0.29) cps] (P<0.01). The number and diameter of adhering granule on the villi surface of fore-gut, mid-gut and hind-gut of tested groups significantly decreased with supplement of xylanase. The height and density of intestine villi of the tested groups were significantly improved. The height, width and density of intestine villi of 0.10% xylanas group were significantly higher than that of control (P<0.01). According to the states of intestine chyme viscosity, villi and microvilli development in this study, we concluded that fore-gut and mid-gut of Tilapia nilotica were the major places of digestion and absorption. The appropriate amount of xylanase added to wheat basal diet was 0.10%. This study firstly investigated the relationship between xylanase and intestine chyme viscosity, as well as the relationship between intestine chyme viscosity and the development of villi and microvilli. From histology aspect, this study also clarified the mechanism of xylanase improving Tilapia nilotica’s growth, and put forward the concept of “fold villi”.17 Refs.In Chinese. Key words: wheat basal diet; xylanase; Tilapia nilotica; chyme viscosity; intestine villi NIE Guo-xing (College of Life Sciences, Henan Normal University, Xinxiang 453007, China), WANG Jun-li, ZHU Ming-wei, ZHOU Hong-qi//J Fish China,2007,31(1).54-61

    • Studies on antibacterial protein of bullfrog serum infected by Aeromonas hydrophila

      2007, 31(1):62-67. DOI: 10.3724/SP.J.1231

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      Abstract:Foundamental Courses of Aquatic Products Science. Studies on antibacterial protein of bullfrog serum infected by Aeromonas hydrophila. To investigate the antibacterial protein in bullfrog (Rana catesbeiana) serum,bullfrogs were injected intraperitoneally with Aeromonas hydrophila suspension with a concentration of 1×107 cells·mL-1. The antibacterial activities of sera from infected bullfrogs in the period of 7 d post-injection were higher than those of control bullfrogs injected i.p. with sterile physiological saline. The serum antibacterial activity of infected bullfrogs peaked at 3 d post-injection and had a significant difference (P <0.05) to the control counterpart at 3 d and 7 d post-injection. Another group of bullfrogs were injected i.p. with Aeromonas hydrophila, and the sera were collected 3 d post-injection. The sera were separated by Sephadex G-25 gel filtration. The results of OD280 showed that there were 2 main peaks of the fractions collected. The antibacterial activity of serum to Aeromonas hydrophila was determined and the results showed that the first peak contained the main antibacterial protein. The antibacterial range of the antibacterial fractions was determined by using 10 strains as indicator, among them, Escherichia coli, Bacillus subtillis, Pseudomonas aeruginosa were inhibited. Result of SDS-PAGE assay showed high-molecular-weight of the antibacterial protein. The results indicated that bullfrog would produce multi-antibacterial-component soon after being infected by Aeromonas hydrophilia; the major antibacterial protein had high-molecular-weights and inhibited some Gram-positive and Gram-negative bacteria. 24 Refs.In Chinese. Key words:Rana catesbeiana; Aeromonas hydrophila;antibacterial protein;antibacterial activity ZOU Wen-zheng(Fisheries College of Jimei University, Xiamen 361021,China), ZHANG Jun-jie, YAN Qing-pi, JI Rong-xing //J Fish China,2007,31(1).62-67

    • Analysis of the resistance of heterosis in Fenneropenaeus chinensis to natural infection with white spot syndrome virus

      2007, 31(1):68-75. DOI: 10.3724/SP.J.00001

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      Abstract:Aquatic Conservation. Analysis of the resistance of heterosis in Fenneropenaeus chinensis to natural infection with white spot syndrome virus. A selected population of the shrimp (Fenneropenaeus chinensis) was tagged, which included 42 full-sib families. A sample of 40 tagged test animals per family was infected by WSSV without hominine help. A week later, the surviving animals were taken out and then the survival rate per family was calculated. The virus positive rate of white-spot syndrome per family was detected by nested-PCR (Polymerase Chain Reaction) for every survivor. Comparison was carried out between pairs of families mainly in terms of the survival rates and the conclusion as follows: 4 families’ survival rates were over 60 percent and 7 families’survival rates were under 20 percent. The analysis of correlation among the indexes indicated that the relationship between the survival rate and the virus positive rate of the first-step and second-step PCR was negative correlation and not significantly different. However, there was positive correlation and significant difference between the virus positive rate of the first-step and the second-step PCR. The analysis was carried out depending on both the survival rate and the virus positive rate and the results as follows: one self-fertilized family of Qingdao population and one hybridized family between the cultured family population of Korea(♂) and Rushan population (♀) were much better than those of the others in resistance to WSSV. There were no significant differences between pairs of cross combinations in the survival rate, which were analysed by independent-sample T test. So the family selection probably was the main method for culturing WSSV-resistant breed. The cross combinations were analysed by multiple comparison method and the result showed: there was difference between the cross combination of the cultured family population of Korea(♂)and Rushan wild population (♀)(56.20%±8.75%) compared with the self-fertilized combinations of the cultured family population of Korea (P<0.05) in resistance to WSSV, which showed the cross combination could get the heterosis. However, there was great difference between the cross combination of the Rushan wild population(♂) and the cultured family population of Korea(♀) compared with their reciprocal hybrids in heterosis. 15 Refs.In Chinese. Key words:Fenneropenaeus chinensis; nested-PCR; white-spot syndrome virus (WSSV) LI Su-hong(Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China), ZHANG Tian-shi, MENG Xian-hong, KONG Jie //J Fish China,2007,31(1).68-75

    • Angiotensin I converting enzyme inhibitory activity of shrimp meat hydrolysate by endogenous enzymes from shrimp head and its antihypertensive effects on spontaneously hypertensive rats

      2007, 31(1):76-84. DOI: 10.3724/SP.J.1231

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      Abstract:Preservation and Processing of Aquatic Products. Angiotensin I-converting enzyme inhibitory activity of shrimp meat hydrolysate by endogenous enzymes from shrimp head and its antihypertensive effects on spontaneously hypertensive rats Hydolysate of shrimp meat hydrolyzed by the endogenous enzymes of the head of Penaeus vannamei, with angiotensin I-converting enzyme (ACE) inhibitory activity of 41.9% (2 mg·mL-1), was prepared under the conditions of pH 7.35, temperature 57.2 ℃, hydrolysis time 4 hours, shrimp head/shrimp meat 1∶1, and substrate concentration 20%. The hydrolysate had a broad molecular weight distribution (between 7.4×104 and 29.7). Fractions with molecular weight under 2.7×103 covered 83.1% of the total hydrolysate. Fractions with molecular weight between 959-338 exerted an inhibitory activity of IC50 0.32 mg·mL-1. Phenylalanine, hydrophobic (Leucine, Isoleucine and Valine ) and aromatic(Proline, Tyrosine and Tryptophan ) amino acids covered 42.33% of the total amino acids of Peak C. Results of the experiments in vivo showed that the hydrolysate exerted a significant antihypertensive activity in spontaneously hypertensive rats after oral administration. 13 Refs.In Chinese. Key words:Penaeus vannamei; endogenous enzymes; angiotensin I-converting enzyme inhibitory activity; antihypertensive effects HONG Peng-zhi( College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China), CAO Wen-hong, HAO Geng-xin, ZHANG Chao-hua//J Fish China,2007,31(1).76-83

    • Study on the characters of tension in mooring lines of anti-stormy-wave-cage with cylindrical net under the combined action of waves and currents

      2007, 31(1):84-89. DOI: 10.3724/SP.J.1231

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    • Effect of light and different forms of nitrogen on the activity of nitrate reductase of Porphyra haitanensis (Rhodophyta)

      2007, 31(1):90-96. DOI: 10.3724/SP.J.1231

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    • >渔业资源与环境专辑
    • A preliminary study on cultivation of the crossbred Porphyra haitanensis thallus and their economic characteristics

      2007, 31(1):97-104. DOI: 10.3724/SP.J.1231

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    • Application of ISSR markers in germplasm identification of different color’s Porphyra haitanensis filament strains

      2007, 31(1):105-111. DOI: 10.3724/SP.J.1231

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    • Selection of a high-temperature resistant strain of Porphyra haitanensis (Rhodophyta)

      2007, 31(1):112-119. DOI: 10.3724/SP.J.00001

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    • >ROUNDUPS
    • Study on spermatophore storage and dehiscence in crab

      2007, 31(1):120-128. DOI: 10.3724/SP.J.00001

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